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Enzo Life Sciences Cyto-ID Autophagy Detection Kit

 

Autophagy is a stress-induced protective mechanism. Less active under basal conditions, the mechanism is used by eukaryotic cells through lysosome-mediated bulk degradation of cellular contents when subjected to certain hostile conditions such as nutrient depletion and chemical or environmental stress. The role of increased autophagic activity in the pathology of cancer, neurodegeneration, cardiovascular disease and diabetes has become widely recognised and commonly studied.

The Cyto-ID Autophagy Detection Kit from Enzo Life Sciences measures autophagic vacuoles and monitors autophagic flux in live cells using a novel dye that selectively labels autophagic vacuoles. The dye has been optimised through the identification of titratable functional moieties that allow for minimal staining of lysosomes while exhibiting bright fluorescence upon incorporation into pre-autophagosomes, autophagosomes and autolysosomes (autophagolysosomes). The assay offers a rapid and quantitative approach to monitoring autophagy in live cells without the need for cell transfection.

Key advantages include: no transfection required; dye includes titratable moieties specific for selectively staining autophagic vesicles; protocol validated with known inhibitors and activators of autophagic activity; rapidly quantifies autophagy in native heterogeneous cell populations; eliminates need for time- and effort-consuming transfection efficiency validation required with LC3-GFP transfection; selective and comprehensive staining allows measurement and differentiation between autophagic flux and autophagolysosome accumulation; negligible staining of lysosomes reduces background seen with other dyes; facilitates high-throughput screening of activators and inhibitors of autophagy.

Each kit contains sufficient reagents for 200 flow cytometry assays, 250 microscopy assays or 3 x 96-well microplate assays.

 

 

 

 

 

GeneCopoeia miProfile miRNA qPCR arrays

 

 

The miProfile human miRNome miRNA qPCR arrays from GeneCopoeia are a set of nineteen 96-well plates covering 1565 of best characterised and annotated miRNAs based on miRBase V17. Each plate contains up to 84 pairs of PCR primers and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process - from reverse transcription to qPCR reaction.

Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template.

Using a universal real-time PCR condition, one can profile and analyse the miRNA expression in a high-throughput fashion. The GeneCopoeia All-in-One miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are suitable RT-PCR reagents for use with the arrays. The reagents are said to produce high sensitivity, efficiency and specificity.

To help distinguish single-nucleotide mismatched miRNAs, 61 single-nucleotide mismatched miRNAs from the human miRNome are further grouped together on one 96-well plate. The miProfile human single-nucleotide mismatch miRNA qPCR array is available as standalone products for users who want to study these miRNAs using specific PCR conditions.

Each miRNA primer is designed using a proprietary algorithm and experimentally validated. It is able to detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA. It is also able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimentally validated for specific amplification.

The product allows miRNA at varieties of expression level to be detected simultaneously. It has high reproducibility (R2>0.99) for inter-array and intra-array replicates.

 

 

 

 

 

Hanby Environmental TPH soil and water kits

 

 

Field TPH soil and water kits, produced by Hanby Environmental, are now available for the first time in Australia. The company’s method offers a solution to obtaining on-site samples that potentially contain petroleum-related substances.

Field testing is inherently costly, time consuming and often unreliable. The Hanby method is designed to provide a precise colour indication of the concentration of petroleum in field samples. The rapid, accurate method has won recognition by the US Environmental Protection Agency, state regulatory agencies throughout the US and various international regulatory agencies around the world.

The field-portable method provides analyses down to ppb for aromatic compounds, including gasoline, diesel, PCBs and many others. The procedure extracts the aromatic compounds from the sample and provides immediate colorimetric identification of the concentration and type of contaminants present. The colour of the reacted extract solution indicates the type of compound and the intensity of the colour indicates the concentration. The results of the test compare consistently from sample to sample over a wide range of concentrations in soil, fresh water and salt water.

The product line functions as a self-contained portable laboratory in a rugged, lightweight briefcase. Each kit has been designed to include all of the materials necessary for non-technical personnel to use in the field with ease.

 

 

 

 

 

LifeSensors UbiQuant Quantitative Ubiquitin ELISA

 

 

The UbiQuant ELISA from LifeSensors is intended for the accurate determination of total ubiquitin - including free ubiquitin, monoubiquitin and polyubiquitin - in biological samples, including cell lysates, tissue homogenates and plasma.

Total ubiquitin is captured in the wells of a pre-coated microtitre plate using a proprietary ubiquitin binding reagent. Unbound protein is removed by washing and then an anti-ubiquitin monoclonal antibody (VU-2) is added. After removing the unbound VU-2, the bound antibody is measured using an enzyme-linked, anti-mouse antibody.

 

 

 

 

 

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